无动物源成分重组细胞因子

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重组小鼠核因子κ B受体活化因子配体 /Recombinant RANKL，Mouse，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM045-5MP	买一赠一	5 ug	￥600.00	现货
CM045-20MP	买一赠一	20 ug	￥1500.00	现货
CM045-100MP	买一赠一	100 ug	￥4500.00	现货
CM045-500MP	买一赠一	500 ug	￥13000.00	请询
CM045-1000MP	买一赠一	1 mg	￥20000.00	请询

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产品简介

背景描述	Receptor activator of NF-κB (RANK) ligand (RANKL) is type II transmembrane protein with an extracellular domain at the carboxy terminus of TNF cytokine superfamily. RANKL is a 19.8 kDa protein containing 317 residues and high expressed in T cells and T cell rich organs, such as thymus and lymph nodes. RANKL-RANK (RANKL receptor) plays an important role in bone metabolism, dysregulation, and immune system. RANKL deficiencies in mice or humans are associated with abnormally increase bone density and blemish in lymphoid organogenesis.
别称	receptor activator of nuclear factor kappa-Β ligand, soluble Receptor Activator of NF-kB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254,sRNAK Ligand
蛋白编码	O35235
分子量	The protein has a calculated MW of 20.35 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED₅₀ for this effect is <2 ng/mL.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant mouse RANKL

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