无动物源成分重组细胞因子

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重组小鼠白细胞介素4 /Recombinant IL-4，Mouse，Animal-Free

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产品编号	优惠促销	规格	价格	货期
CM005-5MP	买一赠一	5 ug	￥600.00	现货
CM005-20MP	买一赠一	20 ug	￥1500.00	现货
CM005-100MP	买一赠一	100 ug	￥4500.00	现货
CM005-500MP	买一赠一	500 ug	￥13000.00	请询
CM005-1000MP	买一赠一	1 mg	￥20000.00	请询

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产品简介

背景描述	Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.
别称	interleukin 4, BCGF, BCDF, B-cell Stimulating Factor (BSF-1), IL4
蛋白编码	P07750
分子量	The protein has a calculated MW of 14.5 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
表达系统	Escherichia coli
纯度	>98% as determined by SDS-PAGE.
生物活性	Measure by its ability to induce HT-2 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-4 is approximately >1 x 10⁶ IU/mg.
内毒素检测	<0.1 EU per 1 μg of the protein by the LAL method.
蛋白序列	MHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS with polyhistidine tag at the C-terminus.
蛋白标签	His Tag (C-term)
产品形式	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
产品应用	Cell Culture

产品储存/运输

储存	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
运输	Blue Ice

产品使用

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

实验数据展示

SDS- PAGE analysis of recombinant mouse IL-4

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