| 背景描述 | R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis. |
| 别称 | RSPO1, Roof plate-specific spondin-1 |
| 蛋白编码 | Q2MKA7 |
| 分子量 | The protein has a calculated MW of 46.8 kDa. The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis). |
| 表达系统 | HEK293 |
| 纯度 | >95% as determined by SDS-PAGE. |
| 生物活性 | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is < 3 μg/mL. |
| 内毒素检测 | <1 EU per 1 μg of the protein by the LAL method. |
| 蛋白序列 | A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus. |
| 蛋白标签 | His-SUMO Tag (N-term) |
| 产品形式 | Liquid (1X PBS, pH 7.4). If you have any concerns or special requirements, please confirm with us. |
| 产品应用 | Cell Culture |
| 浓度 | Please refer to the product label. |
| 储存 | Stock Solution: Store at 2°C to 8°C for up to 1 week, -20°C or -80°C for 12 months. Dilution: Store at -20°C or -80°C for 3 to 6 months with a carrier protein or stabilizer. |
| 运输 | Blue Ice |
1. Before opening, centrifuge at 3000 rpm for 5 mins.
2.Stock Solution
· Store at 2°C to 8°C for up to 1 week, -20°C or -80°C for 12 months.
3.Dilution
· Further dilute the stock solution in a buffer containing a carrier protein or stabilizer (e.g., 0.1% BSA, 10% FBS, 5% HSA, or 5% trehalose solution). The final concentration is not less than 10 μg/ml.
· Prepare aliquots (≥20 μl).
· Store at -20°C or -80°C for 3 to 6 months.
Avoid repeated freeze-thaw cycles.
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| SDS-PAGE analysis of recombinant human R-spondin 1 |
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